Thursday, June 6, 2013

3130 Genetic Analyzer


Description

The Applied Biosystems® 3130 Genetic Analyzer is the latest generation of 4-capillary electrophoresis instruments for the low to medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity.

With the Applied Biosystems® 3130 Genetic Analyzer, you get the sophisticated automation and superior performance of the 3130 system, at acquisition and operating costs tailored to a growing research lab.
• Run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening.
• Reduce maintenance time by eliminating manual syringe washing and filling with automated polymer delivery.
• Increase your data quality for sequencing and fragment analysis applications – longer read length, and higher resolution with shorter run times.
• Increase laboratory productivity and turn around time by processing 96- or 384-well plates with a four-capillary array.

Perform a Wide Range of Applications
The Applied Biosystems 3130 Genetic Analyzer is more than just a DNA sequencer. You can run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening – as well as de novo sequencing and resequencing (mutational profiling). The full range of applications can be run on a single polymer and capillary array meaning you can run mixed applications on one plate. The software even includes tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).

Easy to Use
Reduce maintenance time by eliminating manual syringe washing and filling with the new automated polymer delivery system. No more handling of polymer syringes for set up and maintenance. Samples are automatically injected into the four-capillary arrays, and – unlike slab-gel systems – only minimal amounts of DNA are required for accurate analysis. Seamlessly switch between sequencing and fragment analysis runs, even in the same plate with the expanded one-polymer, one-array functionality for both sequencing and fragment analysis applications.

Superior Data Quality
Simply set up your sample and then sit back and watch as the analyzer's ultra-rapid sequencing gives you high-quality data (with Length of Read longer than 500 bp) in less than 35 minutes. Achieve accurate read lengths of 1,000 base pairs (bp) or longer in a single sequencing reaction using the 80 cm capillary array and the 3130 POP-7™ polymer.

Ideal for Growing Labs
The flexible, four-capillary 3130 system gives you all the advanced automation and superior performance of Applied Biosystems 3130xl platform, at acquisition and operating costs tailored to a growing research lab. And as your throughput needs increase, you can easily upgrade the system to 16 capillaries. It is the perfect way to get the capacity and savings you need today – without limiting your growth options. Researchers who own an ABI PRISM® 3100-Avant or 3100 Genetic Analyzer can upgrade to the new Applied Biosystems® 3130 or 3130xl Genetic Analyzer.

3130 and 3130xl instruments are CE marked and compliant with the specifications and requirements as set in the EMC directive 89⁄336⁄EEC and the Low Voltage directive 73⁄23⁄EEC.

pecifications

General Specifications

Number of Capillaries:
4 Capillaries 
Throughput:
5,760 genotypes/24hr (5-dye DNA sizing), 
4,320 genotypes/24hr (4-dye DNA Sizing), 
30,400 bases/24hr (Long Read Sequencing), 
82,000 bases/24hr (Ultra Rapid Sequencing) 
Performance:
98.5% basecalling accuracy 
Read Length:
Up to 950bp 
Capillary Length:
36cm, 
22cm, 
80cm, 
50 cm 
Compatible Polymers:
POP-7, 
POP-4, 
POP-6 
Polymer Consumption:
Up to 960 samples per 7000µl Bottle 
Platform:
3130 Genetic Analyzer 
Format:
96-well plate, 
384-well plate 
Dimensions:
Width (open): 149cm, 
Height: 81cm, 
Depth: 55cm 
Weight:
130 kg 
Product Size:
1 instrument 
Sample Volume:
> 10µl 
Operating System:
Windows XP Pro 
Current Limit:
15 A 
Plan Duration:
1 Year Warranty 
Voltage Limit:
200-220V or 230-240V 
Analysis Software:
SeqScape® Software, 
Sequencing Analysis Software, 
GeneMapper® Software v4.0, 
Sequence Scanner Software 
Compatible Products:
dRhodamine Dye Terminator Kit, 
BigDye®Terminator Kits 
Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures. 
Operating Environment:
Humidity: 20%-80%, 
Temperature: 15°C-30°C 

Tuesday, June 4, 2013

Miami Heat Eastern Champion

6-3-dwyane-wade-heat-pacers-game-7-gamer-2MIAMI — Dwyane Wade will pay no attention to any criticism of his game as the Miami Heat get ready to play the San Antonio Spurs in the NBA Finals beginning Thursday.
That's because he ignored it going into Game 7 of the Eastern Conference finals and had his best game of the series. It is a big reason the Heat are in their third consecutive Finals.
"Everything that happened in first six games didn't matter to us," Wade said. "It was all about Game 7."
In that Game 7, Wade had 21 points and nine rebounds, was active defensively and looked like his old self as the Heat destroyed the Indiana Pacers 99-76 in an anticlimactic conclusion to what had been a captivating and competitive series.
"We'll enjoy this," Heat coach Erik Spoelstra said, "for a short period of time."

Miami knew what it would get from LeBron James, who finished with what is standard fare for him: 32 points, eight rebounds and four assists.
The question was, what would the Heat get from Wade and Chris Bosh, who had struggled offensively against the defensive-minded Pacers. Bosh didn't have a great scoring game (nine points) but he grabbed eight rebounds, his series high against a team that rendered him ineffective for a majority of the series.
Miami pressured the Pacers at both ends of the court and took control of the game with a 33-16 second quarter and led 52-37 at halftime. James and Wade combined for 16 points and nine rebounds in the third quarter as the Heat extended their lead to 76-55.
"They taught us a lesson," Pacers coach Frank Vogel said. "This team has been there before. They have been to the championship. They've won it all. And they know how to ratchet up their defense at a level that just imposes their will on a basketball game."
James set the tone, first with words to his teammates at shootaround Monday morning and then on the court. As usual, he looked to get others involved. James didn't take one of Miami's first 14 shots,
"The first play of the game I called a play for D-Wade," James said. "Even though he didn't shoot the ball, he got a good touch in the paint. Just to make him feel like he was a part of the offense, make him feel in a good rhythm. I called a couple of sets for him early in the game, just to get a feel for it. And it showed throughout the whole game that he was in the rhythm. He started to make lay-ups, he started to attack, he started to make his free throws. So it was big time."

James' first attempt came with 4:45 left in the first quarter. He then scored 18 points in the final 16 minutes, 18 seconds of the first half.
James attacked the basket and made 15-for-16 free throws and finished with another big Game 7. In four career Game 7s, James is averaging 33.8 points and 8.3 rebounds.
"This is what it's all about," James said. "I've dreamed about opportunities like this as a kid to have a Game 7, no matter at home or on the road. And that game allows you to advance to the Finals.I have had multiple dreams about it."

Pacers forward Paul George emerged as a player with superstar potential, but Miami held him to seven points on 2-for-9 shooting. George fouled out with 7:43 left in the game.
"The great thing is we're a young team and we are past the building stage," George said. "This is really our first year tasting success. The rate we are going, we see championships soon."
The Heat also neutralized the dominance that center Roy Hibbert exhibited at times throughout the series, although he still had 18 points and eight rebounds.
"By any means necessary ... we took care of business," James said. "We just focused on every possession, trying to get stops, play Miami Heat defense, create havoc. I thought we did that tonight."

The Pacers faced a huge task to start. They had to beat the Heat in two consecutive games, including the final game on the road, and the Heat haven't lost consecutive games since January. As confident as coach Frank Vogel was in his team, that's asking a lot.
"Everybody in this country knows who the Indiana Pacers are now," Vogel said. "And we represent all the right things: class, character, hard work, old-school basketball, playing the game the right way. We represented our franchise, our city and our state extremely, extremely well, and we have a lot to be proud of."
Vogel's biggest concern — turnovers — unfurled in the first half: 15 turnovers, including nine in the first quarter.
"We knew that 15 turnovers in a game was probably going to equal a loss tonight. So to do it in a half was dispiriting," Vogel said.

The Heat took advantage of turnovers and missed shots. Miami pushed the ball at every opportunity, beating the Pacers down the court for open shots. Guard Ray Allen, who entered the game shooting 29.2% on three-pointers in the series, made 3-for-4 in the first half.
Miami pressured the Pacers at both ends of the court and took control of the game with a 33-16 second quarter and led 52-37 at halftime. Vogel coached an outstanding series, but there was only so much he could do with the Heat charging.
James and Wade, still bothered by a bruised right knee, combined for 16 points and nine rebounds in the third quarter as the Heat extended their lead to 76-55.
"I'm going to play through pain because this is my job," Wade said. "My team depends on me. Like I said a couple of series ago, I would love to be one of the players who never has to deal with these conversations, never have to deal with these injuries. But that's not my path.
"I've been through so much away from the game and in the game that I'll find a way. I'll figure it out. Some way, some how, you give me enough time, I'll figure it out. That's what I was able to do tonight. That's what I'll hopefully do next season."
The Pacers proved themselves more than a worthy opponent for the Heat. But Miami knew what was stake. Indiana is just learning.
"They just had that killer instinct, that look in their eye that they weren't going to be denied," Vogel said. "Their ball movement was spectacular. That's what really led to a lot of the chain reactions of our defense and allowed enough driving lanes. But they were also relentless in crashing the glass at all positions. Not just their big guys, but they were all coming.
"Look, they just had greater experience and greater know-how, and they were able to reach a higher level than we were."

Saturday, June 1, 2013

IT’S SHOWTIME’S FIRST ULTIMATE KALOKALIKE is christopher de leon

Christopher de leon Kalokalike



Christopher de leon


For his striking resemblance to actor Christopher de Leon, impersonator Jonathan Garcia was named as the Ultimate Kalokalike Saturday (June 1) in ABS-CBN’s noontime show “It’s Showtime’s” look-alike contest grand finals.

 Aside from bearing a close physical resemblance to the veteran actor for which he also won the special Picture Frame Award, Jonathan ultimately clinched the title after showing off his acting chops in the competition’s talent portion.
As the Ultimate Kalokalike, Jonathan will bring home P300,000 and a special celebrity treatment package. He was also awarded the BFF Award, the contest’s equivalent for Mr. Congeniality, for being voted by his co-finalists as the friendliest in the bunch.
Sherylene Castor, Kalokalike of Jinkee Pacquaio, was declared the first runner-up and won P200,000, while Learsi Gomez, Kalokalike of Julia Montes, was named the second runner-up with P100,000.
The Top Five Kalokalikes’ Bottom Two placers were international recording artist Adele and Kapuso host-actor Joey de Leon, who both won P50,000.
Aside from proclaiming the Top Five Kalokalikes that were selected by the panel of judges composed of masterimpersonator Willie Nepumoceno, Jason “Tito Bhoy” Gainza, comedian Pooh, and host Jhong Hilario, “It’s Showtime” also handed out special awards to finalists who made a lasting impact on the madlang people.
The Kalokalike of Justin Bieber was given the Trending Award for generating the wildest buzz on social networking sites, as proven by the Twitter-trending hashtag #CliffHoganAsTheUltimateKalokalike, while Jay Failanga, the Kalokalike of Vice Ganda, clinched the Texters’ Choice Award for garnering the highest number of text votes from the viewers. 
Meanwhile, the Kalokalike of Julia Montes received the Kumarir Award for being the most improved contender in terms of impersonation, wardrobe, and overall look.
The look-alike contest’s grand finals was widely discussed online as the hashtag #UltimateKalokalike topped the list of worldwide trending topics on micro-blogging site Twitter.
Stay tuned to “It’s Showtime,” hosted by Anne Curtis, Vice Ganda, Vhong Navarro, Billy Crawford, Karylle, Kuya Kim Atienza, Teddy Corpuz, Jugs Jugueta, Jhong Hilario, Ryan Bang, Coleen Garcia.